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| Registros recuperados : 45 | |
| Registros recuperados : 45 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/04/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FERREIRA, M. T. M.; ROCHA, L. C.; VITORIANO, M. B. Z.; MITTELMANN, A.; TECHIO, V. H. |
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Afiliação: |
Marco Tulio Mendes Ferreira, UFLA; Laiane Corsini Rocha, UFLA; Matheus Braga Zanon Vitoriano, UFLA; ANDREA MITTELMANN, CNPGL; Vânia Helena Techio, UFLA. |
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Título: |
Relationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, p. 1663-1679, 2018. |
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DOI: |
10.1007/s11033-018-4310-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The grasses of the Lolium?Festuca complex show a prominent role in world agricultural scenario. Several studies have demonstrated that the plasticity of 45S rDNA sites has been recently associated with the possible fragility of the loci. Often, these fragile sites were observed as extended sites and gaps in metaphases. This organization can be evaluated in relation to their transcriptional activity/accessibility through epigenetic changes. Thus, this study aimed to investigate the relationship of the 5-methylcytosine and histone H3 lysine-9 dimethylation in different conformations of 45S rDNA sites in interphase nuclei and in metaphase chromosomes of L. perenne, L. multiflorum and F. arundinacea. The FISH technique using 45S rDNA probes was performed sequentially after the immunolocalization. The sites showed predominantly the following characteristics in the interphase nuclei: intra- and perinucleolar position, decondensed or partially condensed and hypomethylated and hyper/hypomethylated status. Extranucleolar sites were mainly hypermethylated for both epigenetic marks. The 45S rDNA sites with gaps identified in metaphases were always hypomethylated, which justifies it decondensed and transcriptional state. The frequency of sites with hypermethylated gaps was very low. The structural differences observed in these sites are directly related to the assessed epigenetic marks, justifying the different conformations throughout the cell cycle. |
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Palavras-Chave: |
Epigenetic marks; Fragile sites; Lolium Festuca complex; Post translational modification. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02236naa a2200229 a 4500
001 2108357
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-018-4310-9$2DOI
100 1 $aFERREIRA, M. T. M.
245 $aRelationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex.$h[electronic resource]
260 $c2018
520 $aAbstract The grasses of the Lolium?Festuca complex show a prominent role in world agricultural scenario. Several studies have demonstrated that the plasticity of 45S rDNA sites has been recently associated with the possible fragility of the loci. Often, these fragile sites were observed as extended sites and gaps in metaphases. This organization can be evaluated in relation to their transcriptional activity/accessibility through epigenetic changes. Thus, this study aimed to investigate the relationship of the 5-methylcytosine and histone H3 lysine-9 dimethylation in different conformations of 45S rDNA sites in interphase nuclei and in metaphase chromosomes of L. perenne, L. multiflorum and F. arundinacea. The FISH technique using 45S rDNA probes was performed sequentially after the immunolocalization. The sites showed predominantly the following characteristics in the interphase nuclei: intra- and perinucleolar position, decondensed or partially condensed and hypomethylated and hyper/hypomethylated status. Extranucleolar sites were mainly hypermethylated for both epigenetic marks. The 45S rDNA sites with gaps identified in metaphases were always hypomethylated, which justifies it decondensed and transcriptional state. The frequency of sites with hypermethylated gaps was very low. The structural differences observed in these sites are directly related to the assessed epigenetic marks, justifying the different conformations throughout the cell cycle.
653 $aEpigenetic marks
653 $aFragile sites
653 $aLolium Festuca complex
653 $aPost translational modification
700 1 $aROCHA, L. C.
700 1 $aVITORIANO, M. B. Z.
700 1 $aMITTELMANN, A.
700 1 $aTECHIO, V. H.
773 $tMolecular Biology Reports$gv. 45, p. 1663-1679, 2018.
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